Matched antibody pairs consist of two different antibodies generated against the same target, but with different binding regions or epitopes. Differential epitope recognition allows for simultaneous binding of both antibodies to the same target. They can be used in assays such as sandwich ELISA to detect the antigen by immobilizing one antibody on a substrate to capture the antigen while the second antibody is labeled to detect the captured antigen. In some cases, an antibody may self pair.
