Functional protein microarray is a high-throughput technique used to analyze protein interactions, functions, and biochemical properties on a large scale. This method involves immobilizing a diverse array of proteins onto a solid surface, allowing researchers to simultaneously probe interactions with various ligands, antibodies, or other proteins. Functional protein microarrays are particularly valuable for studying protein-protein interactions, enzyme-substrate relationships, and the binding affinities of antibodies or small molecules. By providing a comprehensive overview of protein functions and interactions, this technology accelerates the discovery of novel biomarkers, therapeutic targets, and drug candidates. Additionally, functional protein microarrays are extensively used in fields such as immunology, cancer research, and vaccine development, making them an essential tool in the advancement of both basic research and clinical applications.